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	Provedor de dados BJM (2) Trop. Plant Pathol. (1) Infoteca-e (1) Biol. Res. (1) Bragantia (1) Ciência Rural (1) Mais... Autor AVENDAÑO-HERRERA,RUBÉN (1) Almeida,Irene Maria Gatti de (1) Beriam,Luis Otavio Saggion (1) Bogatzevska,Nevena (1) Ciapina,Luciane P. (1) Costa,Mateus Matiuzzi da (1) Destefano,Suzete Aparecida Lanza (1) Guerreiro Filho,Oliveiro (1) Lemos,Eliana G. M. (1) Lidani,Kárita Cláudia Freitas (1) MAGARIÑOS,BEATRIZ (1) MANCUSO,MONIQUE (1) MEHTA, A. (1) MEHTA, Y. R. (1) Maciel,Karen Wolf (1) Moncheva,Penka (1) Otman,J. (1) Patricio,Flavia Rodrigues Alves (1) Peixoto,Renata de Moraes (1) Peixoto,Rodolfo de Moraes (1) Mais... Palavra-chave REP-PCR (7) RAPD (2) Bacterial spot (1) Coffea arabica (1) Diversidade genética. (1) Doença de Planta (1) Drechslera avenae (1) ERIC- (1) ERIC-PCR (1) ERICPCR (1) Genetic variation (1) Genotipagem (1) Heterogeneity (1) Klebsiella pneumoniae (1) Mancha Parda (1) Mastite (1) Oligonucleotide (1) Ornithinolytic (1) PCR (1) Photobacterium damselae ssp (1) Mais... Tipo do documento Info:eu-repo/semantics/article (5) Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) (1) Journal article (1) Ano 2018 (2) 2013 (1) 2007 (2) 2005 (1) 2003 (1) País Brazil (6) Chile (1) Idioma Inglês (5) Português (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 7 Primeira ... 1 ... Última Atypical phenotypic characteristics of klebsiella pneumoniae isolates from an outbreak in a neonatal intensive care unit in Brazil BJM Otman,J.; Perugini,M.E.; Tognim,M.C.B.; Vidotto,M.C.. Extended-spectrum beta-Lactamase-producing (ESBL) Klebsiella sp.isolates from an outbreak in a Neonatal Intensive Care Unit (NICU) at a teaching hospital in Londrina, Paraná State, Brazil, presented atypical phenotypic characteristics that hampered their identification and the distinction between Klebsiella and Enterobacter species. Ten isolates were identified as K. pneumoniae due to negative reactions for motility and inducible beta-lactamase test (ESBL and AmpC) despite being positive for ornithyne descarboxilase. These isolates were genotyped by ribotyping and polymerase chain reaction (PCR) with repetitive extragenic palindromic sequences (REP). Ribotyping by means of an automated instrument and EcoRI and Pvu II as restriction enzymes resulted... Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; REP-PCR; Genetic variation; Ornithinolytic. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200016 Bacterial halo blight of coffee crop: aggressiveness and genetic diversity of strains Bragantia Maciel,Karen Wolf; Destefano,Suzete Aparecida Lanza; Beriam,Luis Otavio Saggion; Almeida,Irene Maria Gatti de; Patricio,Flavia Rodrigues Alves; Rodrigues,Lucas Mateus Rivero; Guerreiro Filho,Oliveiro. ABSTRACT Bacterial halo blight, caused by Pseudomonas syringae pv. garcae, is an important disease of coffee crop occurring in Brazil and other countries. In recent years, outbreaks of this disease have damaged several coffee crops in Brazil. Aggressiveness and genetic diversity of 25 strains of P. s. pv. garcae, obtained between the years 1958 and 2011, in 23 cities of São Paulo and Minas Gerais states, as well as three strains from Kenya were evaluated in this study. The strains were inoculated on coffee seedlings cultivar Mundo Novo, and their genetic diversity was evaluated by ERIC-PCR, REP-PCR, and their combination. All the strains were pathogenic to the coffee seedlings; the results of pathogenicity tests, in both experiments, could be divided in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas syringae pv. garcae; Coffea arabica; ERICPCR; REP-PCR. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000100096 Development of a scar marker for Pierce's Disease strains of Xylella fastidiosa Trop. Plant Pathol. Travensolo,Regiane F.; Ciapina,Luciane P.; Lemos,Eliana G. M.. The objective of this research was to develop a primer for a polymerase chain reaction specific for Xylella fastidiosa strains that cause Pierce's Disease (PD) in grapes (Vitis vinifera). The DNA amplification of 23 different strains of X. fastidiosa, using a set of primers REP1-R (5'-IIIICGICGIATCCIGGC-3') and REP 2 (5'-ICGICTTATCIGGCCTAC-3') using the following program: 94 ºC/2 min; 35 X (94 ºC/1 min, 45 ºC/1 min and 72 ºC/1 min and 30 s) 72 ºC/5 min, produced a fragment of 630 bp that differentiated the strains that cause disease in grapes from the other strains. However, REP banding patterns could not be considered reliable for detection because the REP1-R and REP 2 primers correspond to repetitive sequences, which are found throughout the bacterial... Tipo: Info:eu-repo/semantics/article Palavras-chave: Oligonucleotide; Polymerase chain reaction; PCR; REP-PCR. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200002 Diversidade genética em Drechslera avenae Stemphylium solani utilizando ERIC- e REP-PCR. Infoteca-e MEHTA, A.; MEHTA, Y. R.; ROSATO, Y. B.. bitstream/CENARGEN/24690/1/bp047.pdf Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Drechslera avenae; ERIC-; REP-PCR; Diversidade genética.; Doença de Planta; Mancha Parda; Stemphylium Solani.. Ano: 2003 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/185432 Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida Biol. Res. MANCUSO,MONIQUE; AVENDAÑO-HERRERA,RUBÉN; ZACCONE,R; TORANZO,ALICIA E; MAGARIÑOS,BEATRIZ. This study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp. piscicida strains isolated from different species of marine fish and geographic areas. The results obtained by the three methods showed that RAPD and ERIC-PCR were more discriminative for suitable rapid typing of Ph. damselae ssp. piscicida than REP-PCR. The analysis of DNA banding patterns generated by both molecular methods (RAPD and ERIC-PCR) clearly separated the strains into two main groups that strongly correlated with their geographic origin. Moreover,... Tipo: Journal article Palavras-chave: Photobacterium damselae ssp; Piscicida; RAPD; ERIC-PCR; REP-PCR. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100009 Genotipificação de isolados de Staphylococcus epidermidis provenientes de casos de mastite caprina Ciência Rural Peixoto,Renata de Moraes; Peixoto,Rodolfo de Moraes; Lidani,Kárita Cláudia Freitas; Costa,Mateus Matiuzzi da. Para verificar a dinâmica da resistência aos antimicrobianos em uma propriedade rural no município de Santa Maria da Boa Vista, PE, foram avaliados 14 isolados de Staphylococcus epidermidis de caprinos com mastite subclínica. O perfil de resistência aos antimicrobianos foi determinado pelo teste de difusão em disco. A genotipificação foi realizada empregando o marcador REP (Repetitive Extragenic Palindromic) - PCR, utilizando o primer RW3A, enquanto os graus de similaridade e o fenograma de agrupamento foram estabelecidos por meio do coeficiente de Sorensen-Dice (SD) do algoritmo UPGMA, programa NTSYS-pc, o qual permitiu a identificação de 4 padrões dos 14 isolados de S. epidermidis, sendo oito no perfil A, quatro no perfil B, um no perfil C e um no perfil... Tipo: Info:eu-repo/semantics/article Palavras-chave: Genotipagem; Mastite; REP-PCR; Staphylococcus epidermidis. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000200021 Molecular methods for diversity assessment among xanthomonads of Bulgarian and Macedonian pepper BJM Vancheva,Taca; Stoyanova,Mariya; Tasheva-Terzieva,Elena; Bogatzevska,Nevena; Moncheva,Penka. ABSTRACT Bacterial spot is an important disease of pepper in Bulgaria and Macedonia. For characterization of Xanthomonas species associated with bacterial spot, 161 strains were collected from various field pepper-growing regions. Among them, 131 strains were identified as Xanthomonas euvesicatoria and 30 as Xanthomonas vesicatoria using species-specific primers and polymerase chain reaction followed by restriction fragment length polymorphism analysis. To assess the genetic diversity of the strains, two methods (Random Amplified Polymorphic DNA and Repetitive Element Palindromic-Polymerase Chain Reaction) were applied. Discriminatory index was calculated and analysis of molecular variance was carried out.Combined random amplified polymorphic DNA analysis... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial spot; RFLP; RAPD; REP-PCR; Heterogeneity. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500246 Registros recuperados: 7 Primeira ... 1 ... Última

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